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Chitosan-assisted permeabilization of ATP–biotin for live cell kinase-catalyzed biotinylation
Kinases are essential cell signaling enzymes that phosphorylate protein substrates using ATP as the universal cosubstrate. A wide variety of ATP analogs have been used in kinase research, although the studies are limited by the cell impermeability of ATP. Here we describe the use of the cationic pol...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2018-09-01
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| Colecção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/btn-2018-0076 |
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