The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate

SNAP-tag® is a powerful technology for the labelling of protein/enzymes by using benzyl-guanine (BG) derivatives as substrates. Although commercially available or ad hoc produced, their synthesis and purification are necessary, increasing time and costs. To address this limitation, here we suggest a...

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Detalhes bibliográficos

Principais autores:	Rosa Merlo, Diego Caprioglio, Michele Cillo, Anna Valenti, Rosanna Mattossovich, Castrese Morrone, Alberto Massarotti, Franca Rossi, Riccardo Miggiano, Antonio Leonardi, Alberto Minassi, Giuseppe Perugino
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Taylor & Francis Group 2021-01-01
coleção:	Journal of Enzyme Inhibition and Medicinal Chemistry
Assuntos:	protein-tag protein labelling enzymatic reaction click chemistry biotechnology
Acesso em linha:	http://dx.doi.org/10.1080/14756366.2020.1841182
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