A Pipeline for Faecal Host DNA Analysis by Absolute Quantification of LINE-1 and Mitochondrial Genomic Elements Using ddPCR

Abstract Stool contains DNA shed from cells of the gastrointestinal (GI) tract and has great potential as a bio-specimen for non-invasive, nucleic acid-based detection of GI diseases. Whereas methods for studying faecal microbiome DNA are plentiful, there is a lack of well-characterised procedures f...

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Hlavní autoři:	Kuang He, Hideaki Fujiwara, Cynthia Zajac, Erin Sandford, Pavan Reddy, Sung Won Choi, Muneesh Tewari
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Nature Portfolio 2019-04-01
Edice:	Scientific Reports
On-line přístup:	https://doi.org/10.1038/s41598-019-41753-6
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