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RNA isolation method for single embryo transcriptome analysis in zebrafish

<p>Abstract</p> <p>Background</p> <p>Transcriptome analysis during embryogenesis usually requires pooling of embryos to obtain sufficient RNA. Hence, the measured levels of gene-expression represent the average mRNA levels of pooled samples and the biological variation among individuals is confounde...

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Autori principali: Spaink Herman P, Verkooijen Jurgo, Bruning Oskar, Rauwerda Han, de Jong Mark, Breit Timo M
Natura: Artigo
Lingua:Inglês
Pubblicazione: BMC 2010-03-01
Serie:BMC Research Notes
Accesso online:http://www.biomedcentral.com/1756-0500/3/73
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