Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
Gene tagging with fluorescent proteins is commonly applied to investigate the localization and dynamics of proteins in their cellular environment. Ideally, a fluorescent tag is genetically inserted at the endogenous locus at the N- or C- terminus of the gene of interest without disrupting regulatory...
Guardat en:
| Autors principals: | , , , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Public Library of Science (PLoS)
2016-01-01
|
| Col·lecció: | PLoS ONE |
| Accés en línia: | http://europepmc.org/articles/PMC5063382?pdf=render |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|