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Oligomerization of the E. coli core RNA polymerase: formation of (α2ββ'ω)2-DNA complexes and regulation of the oligomerization by auxiliary subunits.

In this work, using multiple, dissimilar physico-chemical techniques, we demonstrate that the Escherichia coli RNA polymerase core enzyme obtained through a classic purification procedure forms stable (α(2)ββ'ω)(2) complexes in the presence or absence of short DNA probes. Multiple control experiment...

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Autori principali: Seema G Kansara, Maxim V Sukhodolets
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science (PLoS) 2011-04-01
Serie:PLoS ONE
Accesso online:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018990&type=printable
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