Oligomerization of the E. coli core RNA polymerase: formation of (α2ββ'ω)2-DNA complexes and regulation of the oligomerization by auxiliary subunits.
In this work, using multiple, dissimilar physico-chemical techniques, we demonstrate that the Escherichia coli RNA polymerase core enzyme obtained through a classic purification procedure forms stable (α(2)ββ'ω)(2) complexes in the presence or absence of short DNA probes. Multiple control experiment...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
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Public Library of Science (PLoS)
2011-04-01
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| Schriftenreihe: | PLoS ONE |
| Online-Zugang: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018990&type=printable |
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