Separation and Visualization of Low Abundant Ubiquitylated Forms
In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a membrane-enriched fraction, solubilize the membranes using detergent and...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Bio-protocol LLC
2018-11-01
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| Serie: | Bio-Protocol |
| Accesso online: | https://bio-protocol.org/en/bpdetail?id=3081&type=0 |
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