Impact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells

Abstract The human embryonal kidney 293 cell (HEK‐293) is a widely used expression host for transient gene expression. The genes or plasmids used for the transient transfections are usually propagated and extracted from the gram‐negative bacterium Escherichia coli, the workhorse for molecular biolog...

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Detalhes bibliográficos

Principais autores:	Christine Faust, Christian Beil, Werner Dittrich, Ercole Rao, Thomas Langer
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Maximum Academic Press 2021-11-01
coleção:	Engineering in Life Sciences
Assuntos:	endotoxin HEK‐293 cells lipopolysaccharides recombinant protein expression transient transfection
Acesso em linha:	https://doi.org/10.1002/elsc.202100065
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