Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

Accumulation of proline is a widespread plant response to a broad range of environmental stress conditions including salt and osmotic stress. Proline accumulation is achieved mainly by upregulation of proline biosynthesis in the cytosol and by inhibition of proline degradation in mitochondria. Chang...

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Principais autores:	Sandrine Lebreton, Cécile Cabassa-Hourton, Arnould Savouré, Dietmar Funck, Giuseppe Forlani
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Frontiers Media S.A. 2020-12-01
coleção:	Frontiers in Plant Science
Assuntos:	proline dehydrogenase pyrroline-5-carboxylate reductase enzyme activity assay electron acceptor protein extraction mitochondria
Acesso em linha:	https://www.frontiersin.org/articles/10.3389/fpls.2020.602939/full
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