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Simple, time-saving dye staining of proteins for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue.

A fixation-free and fast protein-staining method for sodium dodecyl sulfate-polyacrylamide gel electrophoresis using Coomassie blue is described. The protocol comprises staining and quick washing steps, which can be completed in 0.5 h. It has a sensitivity of 10 ng, comparable with that of conventio...

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Bibliografski detalji
Glavni autori: Wei-Hua Dong, Tian-Yun Wang, Fang Wang, Jun-He Zhang
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science (PLoS) 2011-01-01
Serija:PLoS ONE
Online pristup:http://europepmc.org/articles/PMC3151240?pdf=render
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