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DNA and RNA Extraction and Quantitative Real-Time PCR-Based Assays for Biogas Biocenoses in an Interlaboratory Comparison

Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA extrac...

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Principais autores: Michael Lebuhn, Jaqueline Derenkó, Antje Rademacher, Susanne Helbig, Bernhard Munk, Alexander Pechtl, Yvonne Stolze, Steffen Prowe, Wolfgang H. Schwarz, Andreas Schlüter, Wolfgang Liebl, Michael Klocke
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI AG 2016-01-01
coleção:Bioengineering
Assuntos:
ring-trial
fermenter sludge
DNA purity
PCR suitability
extraction efficiency
RNA preservation
reverse transcription
methanogens
Bacteria
absolute quantification
Acesso em linha:http://www.mdpi.com/2306-5354/3/1/7
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