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Preparation and Purification of Active Recombinant Human Pancreatic Lipase in Escherichia coli

Human pancreatic lipase (HPL) is the main lipolytic enzyme involved in the digestion of dietary fat. An active recombinant human pancreatic lipase (recHPL) was successfully prepared for the first time in an Escherichia coli (E. coli) expression system using a short Strep-tag II (ST II). The recHPL-S...

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Bibliografiske detaljer
Principais autores: Nanami Kawaguchi, Haruko Ogawa, Kimie Date
Format: Artigo
Sprog:Inglês
Udgivet: Bio-protocol LLC 2019-07-01
Serier:Bio-Protocol
Online adgang:https://bio-protocol.org/en/bpdetail?id=3286&type=0
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