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A Cre-based double fluorescence indicator system for monitoring cell fusion events and selection of fused cells

We have established an in vitro Cre/loxP-based assay for monitoring cell fusion events that specifically traces the transport of cytoplasm from one cell to its fusion partner. Cells with a double fluorescence vector indicate fusion with cells expressing Cre recombinase by switching expression from r...

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Autores principales: Kurt Pfannkuche, Dimitry Spitkovsky, Frank Thomas Wunderlich, Osama Mohamed Abd El Aziz, Tomo Saric, Jürgen Hescheler, Agapios Sachinidis
Formato: Artigo
Lenguaje:Inglês
Publicado: Taylor & Francis Group 2010-02-01
Colección:BioTechniques
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Acceso en línea:https://www.future-science.com/doi/10.2144/000113352
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