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Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification

<p>Abstract</p> <p>Background</p> <p>In this study we utilized padlock probes and rolling circle amplification as a mean to detect and study the replication of porcine circovirus type 2 (PCV2) in cultured cells and in infected tissue. Porcine circovirus type 2 is a single-stranded circular DNA virus...

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Bibliografiske detaljer
Principais autores: Berg Mikael, Fossum Caroline, Fuxler Lisbeth, Blomström Anne-Lie, Henriksson Sara, Nilsson Mats
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2011-01-01
Serier:Virology Journal
Online adgang:http://www.virologyj.com/content/8/1/37
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