Large‐scale image‐based profiling of single‐cell phenotypes in arrayed CRISPR‐Cas9 gene perturbation screens

Abstract High‐content imaging using automated microscopy and computer vision allows multivariate profiling of single‐cell phenotypes. Here, we present methods for the application of the CISPR‐Cas9 system in large‐scale, image‐based, gene perturbation experiments. We show that CRISPR‐Cas9‐mediated ge...

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Auteurs principaux: Reinoud de Groot, Joel Lüthi, Helen Lindsay, René Holtackers, Lucas Pelkmans
Format: Artigo
Langue:Inglês
Publié: Springer Nature 2018-01-01
Collection:Molecular Systems Biology
Sujets:
arrayed library
CRISPR‐Cas9
functional genomics
nuclear pore complex
single‐cell phenotypic profiling
Accès en ligne:https://doi.org/10.15252/msb.20178064
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