Identification of Nitric Oxide Synthase Neurons for Laser Capture Microdissection and mRNA Quantification
An immunohistochemical technique was developed to visualize nitric oxide synthase (NOS)-immunopositive neurons in fresh-frozen tissue sections of rat brain for laser capture microdissection (LCM) and mRNA analysis. The effect of tissue fixation and the choice of fluorophore were investigated. Here w...
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| Principais autores: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis Group
2002-12-01
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| coleção: | BioTechniques |
| Acesso em linha: | https://www.future-science.com/doi/10.2144/02336rr01 |
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