Optimization of coffee (Coffea arabica) transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance
The aim of this work was to optimize the biolistic delivery parameters that affect the DNA delivery and stable expression of marker genes into coffee tissues (Coffea arabica. L. cvs. Caturra and Catuaí). The effect of osmotic preculture length, osmotic concentration of medium, Helium pressure and ta...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Universidad de Costa Rica
2009-11-01
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| Collection: | Revista de Biología Tropical |
| Sujets: | |
| Accès en ligne: | http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442009000500014&lng=en&tlng=en |
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