Microfluidic-Based Amplification-Free Bacterial DNA Detection by Dielectrophoretic Concentration and Fluorescent Resonance Energy Transfer Assisted in Situ Hybridization (FRET-ISH)

Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times. The assay described in this work incorporates on-chip dielectrophoretic capture and concentra...

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Principais autores:	Maxim Shusteff, Evangelyn C. Alocilja, Michelle M. Packard
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2012-10-01
coleção:	Biosensors
Assuntos:	fluorescence resonance energy transfer assisted in situ hybridization (FRET-ISH) in situ hybridization (ISH) dielectrophoresis (DEP) on-chip diagnostics lab on a chip microbial identification
Acesso em linha:	http://www.mdpi.com/2079-6374/2/4/405
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