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Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing

Abstract Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study. Sperm DNA fragmentation, progressive motility, and...

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Автори: Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao
Формат: Artigo
Мова:Inglês
Опубліковано: Wiley 2024-01-01
Серія:Reproductive Medicine and Biology
Предмети:
density gradient centrifugation
in situ nick‐end labeling
sperm DNA fragmentation
sperm progressive motility
swim‐up
Онлайн доступ:https://doi.org/10.1002/rmb2.12562
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