Optimization of a genetically encoded fluorescent sensor for the detection of 5-HT

Objective·To optimize iSeroSnFR, a genetically encoded 5-hydroxytryptamine (5-HT) fluorescent sensor based on bacterial periplasmic binding proteins (PBPs), to enhance its performance for both in vivo and in vitro 5-HT detection.Methods·iSeroSnFR1.2 was engineered by replacing the circularly permute...

ver descrição completa

Detalhes bibliográficos

Principais autores:	XU Mufan, ZHANG Kun, WANG Jingyi, GAO Xinke, CHENG Aobing, ZHANG Peng
Formato:	Artigo
Idioma:	Chinês
Publicado em:	Editorial Office of Journal of Shanghai Jiao Tong University (Medical Science) 2025-05-01
coleção:	Shanghai Jiaotong Daxue xuebao. Yixue ban
Assuntos:	genetically encoded sensor 5-hydroxytryptamine (5-ht) circularly permuted superfolder gfp periplasmic binding protein
Acesso em linha:	https://xuebao.shsmu.edu.cn/article/2025/1674-8115/1674-8115-2025-45-5-529.shtml
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!