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High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay

Summary: High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput screening and sorting of antibody-secreting cells using a Förster resonance electron transfer (FRET)-based assay. The...

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Principais autores: Justina Rutkauskaite, Simon Berger, Stavros Stavrakis, Oliver Dressler, John Heyman, Xavier Casadevall i Solvas, Andrew deMello, Linas Mazutis
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2022-07-01
Serier:iScience
Fag:
Immunology
Biological sciences
Immunological methods
Biotechnology
Online adgang:http://www.sciencedirect.com/science/article/pii/S2589004222007866
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