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Selection of scFv Phages on Intact Cells Under Low pH Conditions Leads to a Significant Loss of Insert-Free Phages

Display of functional antibody fragments on the surface of filamentous bacteriophages allows fast selection of specific phage antibodies against a variety of target antigens. However, enrichment of single chain variable fragment (scFv)-displaying phages is often hampered by the abundance of bacterio...

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Autors principals: M.K. Tur, M. Huhn, S. Sasse, A. Engert, S. Barth
Format: Artigo
Idioma:Inglês
Publicat: Taylor & Francis Group 2001-02-01
Col·lecció:BioTechniques
Accés en línia:https://www.future-science.com/doi/10.2144/01302rr04
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