Supercharged cellulases show superior thermal stability and enhanced activity towards pretreated biomass and cellulose

Non-productive binding of cellulolytic enzymes to various plant cell wall components, such as lignin and cellulose, necessitates high enzyme loadings to achieve efficient conversion of pretreated lignocellulosic biomass to fermentable sugars. Protein supercharging was previously employed as one of t...

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Bibliografische Detailangaben

Hauptverfasser:	Bhargava Nemmaru, Jenna Douglass, John M. Yarbrough, Antonio DeChellis, Srivatsan Shankar, Alina Thokkadam, Allan Wang, Shishir P. S. Chundawat
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2024-07-01
Schriftenreihe:	Frontiers in Energy Research
Schlagworte:	cellulase carbohydrate-binding module computational protein design thermal stability enzyme supercharging lignin inhibition
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fenrg.2024.1372916/full
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