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Unbiased profiling of CRISPR RNA-guided transposition products by long-read sequencing

Abstract Bacterial transposons propagate through either non-replicative (cut-and-paste) or replicative (copy-and-paste) pathways, depending on how the mobile element is excised from its donor source. In the well-characterized E. coli transposon Tn7, a heteromeric TnsA-TnsB transposase directs cut-an...

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Principais autores: Phuc Leo H. Vo, Christopher Acree, Melissa L. Smith, Samuel H. Sternberg
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2021-06-01
Serier:Mobile DNA
Fag:
CRISPR-Cas
RNA-guided transposase
Tn7
Transposition
SMRT-seq
CAST
Online adgang:https://doi.org/10.1186/s13100-021-00242-2
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