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Real-Time PCR Assay for Rapid and Simultaneous Detection of <i>vanA</i> and <i>vanB</i> Genes in Clinical Strains

Here, we develop a robust and sensitive real-time PCR assay which allows the simultaneous detection of <i>vanA</i> and <i>vanB</i> genes using common primers. The system was designed using the Primer3 online software. The specificity of primers and probes was first checked by in silico PCR and by Bl...

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Principais autores: Hanane Zerrouki, Sid-Ahmed Rebiahi, Linda Hadjadj, Jean-Marc Rolain, Seydina M. Diene
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2021-11-01
Serier:Diagnostics
Fag:
real-time PCR assay
simultaneous detection
<i>Enterococci</i>
<i>vanA</i>
<i>vanB</i>
Online adgang:https://www.mdpi.com/2075-4418/11/11/2081
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