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Production of porcine reproductive and respiratory syndrome virus (PRRSV)-resistant genomeedited cloned pigs using CRISPR/Cas9n system

Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of porcine fibroblasts. By flow cytometry, the c...

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Bibliografiske detaljer
Principais autores: WANG Shaohua, ZHAO Panpan, LIU Tong, DING Biao, LUO Lei, CAO Zubing, ZHANG Yunhai, ZHANG Kun
Format: Artigo
Sprog:Inglês
Udgivet: Zhejiang University Press 2018-03-01
Serier:浙江大学学报. 农业与生命科学版
Fag:
Online adgang:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.03.013
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