Production of porcine reproductive and respiratory syndrome virus (PRRSV)-resistant genomeedited cloned pigs using CRISPR/Cas9n system
Using gene editing technology based on CRISPR/Cas9n system and somatic cell nuclear transfer technology, we constructed a plasmid of two sgRNAs and Cas9 nickase, and green fluorescent protein (GFP) in the same vector followed by transient transfection of porcine fibroblasts. By flow cytometry, the c...
Na minha lista:
| Principais autores: | , , , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Zhejiang University Press
2018-03-01
|
| Serier: | 浙江大学学报. 农业与生命科学版 |
| Fag: | |
| Online adgang: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.03.013 |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|
