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Culture, qPCR, and genome-based surveillance of blaKPC, blaNDM, and blaVIM in school wastewater from Santiago, Chile

BackgroundAntimicrobial resistance (AMR) surveillance remains predominantly centered on clinical settings, potentially underestimating the community circulation of high-priority carbapenemase determinants.MethodsWe integrated culture-based isolation, absolute qPCR, and whole-genome sequencing (WGS)...

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Principais autores: Andrés Cortez-Astorga, Diego Lira-Velásquez, Richard Covarrubia-López, Juan Castro-Severyn, Gabriel I. Krüger, Nicolás Pacheco, Aldo Gaggero, Francisco Remonsellez, Sofía Quintana, Felipe Vásquez-Ponce, Claudia P. Saavedra, Jorge Olivares-Pacheco
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2026-04-01
Serier:Frontiers in Microbiology
Fag:
AMR
blaKPC
blaNDM
blaVIM
carbapenemase genes
Escherichia coli ST1193
Online adgang:https://www.frontiersin.org/articles/10.3389/fmicb.2026.1787083/full
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