An in vitro assay to quantify satellite cell activation using isolated mouse myofibers

Summary: Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using s...

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Autors principals:	Rodrigo Canibano-Fraile, Emma Boertjes, Stela Bozhilova, W.W.M. Pim Pijnappel, Gerben J. Schaaf
Format:	Artigo
Idioma:	Inglês
Publicat:	Elsevier 2021-06-01
Col·lecció:	STAR Protocols
Matèries:	Cell isolation Cell-based Assays Microscopy Stem Cells
Accés en línia:	http://www.sciencedirect.com/science/article/pii/S2666166721001891
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