Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1
Especial conditions were developed for the amplification of five DNA segments from US region of BHV-1 by polymerase chain reaction. In order to eliminate most nonspecific products it was found that addition of three cosolvents DMSO, glycerol and NP 40 was a simple method for increasing the specifici...
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| Format: | Artigo |
| Sprache: | Inglês |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-04-01
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| Schriftenreihe: | Memorias do Instituto Oswaldo Cruz |
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| Online-Zugang: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000200015 |
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