Partial CviJI Digestion as an Alternative Approach to Generate Cosmid Sublibraries for Large-Scale Sequencing Projects
We demonstrate an alternative method for the generation of random subclones for large-scale shotgun human DNA sequencing projects. Miniprep DNA from a human cosmid clone was partially digested with CviJI, size-fractionated by agarose gel electrophoresis and cloned into bacteriophage M13. A library c...
Sparad:
| Huvudupphov: | , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Utgiven: |
Taylor & Francis Group
1996-07-01
|
| Serie: | BioTechniques |
| Länkar: | https://www.future-science.com/doi/10.2144/96211st04 |
| Taggar: |
Inga taggar, Lägg till första taggen!
|