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SICyLIA-cTMT dissects redox proteome dynamics with high accuracy and depth at microgram scale

Summary: Cysteine oxidative modifications are critical signaling events regulating cellular functions, but their low abundance and dynamic nature pose technical challenges. We developed the SICyLIA-TMT workflow, which sequentially labels reduced and reversibly oxidized cysteines with light and heavy...

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Principais autores: Sergio Lilla, Samuel Atkinson, Sonja Radau, Ulla-Maja Bailey, Atul Shahaji Deshmukh, Jiska van der Reest, Joanna Kirkpatrick, Thomas MacVicar, Sara Zanivan
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2025-11-01
coleção:Cell Reports: Methods
Assuntos:
CP: biotechnology
Acesso em linha:http://www.sciencedirect.com/science/article/pii/S2667237525002462
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