Mito-kaede photoactivation and chase experiment for mitophagy: optimizing flux measurement via fluid exchange system

Modulating autophagy and mitophagy, vital cellular quality control systems, offer therapeutic potential for critical illnesses. However, limited drug screening options hinder progress. We present a novel assay using the photoswitchable fluorescent reporter, mito-Kaede, to quantify mitophagy flux. Mi...

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Hlavní autoři:	Hiroyuki Morinaga, Yoh Sugawara, Yoshinori Kitagawa, Jingyuan Chen, Nobuo Yasuda, Hiroki Ogata, Yoshihiro Yamaguchi, Masao Kaneki, Joseph A Jeevendra Martyn, Shingo Yasuhara
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis Group 2024-08-01
Edice:	BioTechniques
Témata:	assay autophagy CCCP fluorescent microscopy mito-Kaede mitophagy
On-line přístup:	https://www.tandfonline.com/doi/10.1080/07366205.2024.2372955
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