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3D molecular phenotyping of cleared human brain tissues with light-sheet fluorescence microscopy

An optimized clearing approach, named SHORT, applied to thick sections of human cortical tissue allows reconstruction of large portions of the tissues using light-sheet fluorescence microscopy, by performing multicolor immunostaining and multirounds.

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Príomhchruthaitheoirí: Luca Pesce, Marina Scardigli, Vladislav Gavryusev, Annunziatina Laurino, Giacomo Mazzamuto, Niamh Brady, Giuseppe Sancataldo, Ludovico Silvestri, Christophe Destrieux, Patrick R. Hof, Irene Costantini, Francesco S. Pavone
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Nature Portfolio 2022-05-01
Sraith:Communications Biology
Rochtain ar líne:https://doi.org/10.1038/s42003-022-03390-0
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