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Low-Density Granulocyte Contamination From Peripheral Blood Mononuclear Cells of Patients With Sepsis and How to Remove It – A Technical Report

Elucidating the mechanisms contributing to the dysregulated host response to infection as part of the syndrome is a current challenge in sepsis research. Peripheral blood mononuclear cells are widely used in immunological studies. Density gradient centrifugation, a common method, is of limited use f...

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Principais autores: Judith Schenz, Manuel Obermaier, Sandra Uhle, Markus Alexander Weigand, Florian Uhle
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2021-08-01
Serier:Frontiers in Immunology
Fag:
cell isolation
centrifugation
Ficoll
MACS
density gradient
Sepsis
Online adgang:https://www.frontiersin.org/articles/10.3389/fimmu.2021.684119/full
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