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Internal and external normalization of nascent RNA sequencing run-on experiments

Abstract In experiments with significant perturbations to transcription, nascent RNA sequencing protocols are dependent on external spike-ins for reliable normalization. Unlike in RNA-seq, these spike-ins are not standardized and, in many cases, depend on a run-on reaction that is assumed to have co...

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Bibliográfalaš dieđut
Váldodahkkit: Zachary L. Maas, Robin D. Dowell
Materiálatiipa: Artigo
Giella:Inglês
Almmustuhtton: BMC 2024-01-01
Ráidu:BMC Bioinformatics
Fáttát:
Liŋkkat:https://doi.org/10.1186/s12859-023-05607-3
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