Rare allele enrichment and detection by allele-specific PCR, competitive probe blocking, and melting analysis

Differential amplification of variant and wild-type alleles by PCR is often used for rare allele enrichment. We have combined allele-specific PCR, competitive probe blocking, asymmetric PCR, and melting analysis to enhance rare allele detection in a homogeneous system. Unlabeled, dual hybridization...

Ausführliche Beschreibung

Bibliografische Detailangaben

Hauptverfasser:	Luming Zhou, Ying Wang, Carl T. Wittwer
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis Group 2011-05-01
Schriftenreihe:	BioTechniques
Schlagworte:	Allele-specific competitive blocking PCR melting analysis rare allele enrichment BRAF
Online-Zugang:	https://www.future-science.com/doi/10.2144/000113668
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