Design and Validation of a Peptide Nucleic Acid Clamp of Barley and Wheat ITS2 for Fungal Microbiome Surveys
Next-generation sequencing surveys of plant microbiomes often experience considerable loss of reads due to nontarget amplification of host DNA. To alleviate this, peptide nucleic acid (PNA) clamps can be included during PCR amplification to specifically bind and block amplification of host DNA. Howe...
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| Autor principal: | |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Phytopathological Society
2026-02-01
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| coleção: | PhytoFrontiers |
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| Acesso em linha: | https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-07-25-0066-SC |
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