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Microsatellite Analysis Using a Two-Step Procedure for Fluorescence Labeling of PCR Products

A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a “linear amplification”. This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to label the pro...

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Principais autores: Sophie Jouquand, Angélique Chéron, Francis Galibert
格式: Artigo
語言:Inglês
出版: Taylor & Francis Group 1999-05-01
叢編:BioTechniques
在線閱讀:https://www.future-science.com/doi/10.2144/99265st03
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