Quantification of anti-aggregation activity of chaperones: a test-system based on dithiothreitol-induced aggregation of bovine serum albumin.

The methodology for quantification of the anti-aggregation activity of protein and chemical chaperones has been elaborated. The applicability of this methodology was demonstrated using a test-system based on dithiothreitol-induced aggregation of bovine serum albumin at 45°C as an example. Methods fo...

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Principais autores:	Vera A Borzova, Kira A Markossian, Dmitriy A Kara, Natalia A Chebotareva, Valentina F Makeeva, Nikolay B Poliansky, Konstantin O Muranov, Boris I Kurganov
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Public Library of Science (PLoS) 2013-01-01
coleção:	PLoS ONE
Acesso em linha:	http://europepmc.org/articles/PMC3769246?pdf=render
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