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Single-molecule tracking for studying protein dynamics and target-search mechanism in live cells of S. cerevisiae

Summary: Single-molecule tracking (SMT) is a powerful approach to quantify the biophysical parameters of protein dynamics in live cells. Here, we describe a protocol for SMT in live cells of the budding yeast Saccharomyces cerevisiae. We detail how to genetically engineer yeast strains for SMT, how...

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Ngā kaituhi matua: Nitesh Kumar Podh, Ayan Das, Partha Dey, Sheetal Paliwal, Gunjan Mehta
Hōputu: Artigo
Reo:Inglês
I whakaputaina: Elsevier 2022-12-01
Rangatū:STAR Protocols
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Urunga tuihono:http://www.sciencedirect.com/science/article/pii/S2666166722007808
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