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Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins

Abstract Background In the present work, we provide an account of structured illumination microscopy (SIM) imaging of fixed and immunolabeled plant probes. We take advantage of SIM, to superresolve intracellular structures at a considerable z-range and circumvent its low temporal resolution capacity...

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Principais autores: T. Vavrdová, O. Šamajová, P. Křenek, M. Ovečka, P. Floková, R. Šnaurová, J. Šamaj, G. Komis
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2019-03-01
Serier:Plant Methods
Fag:
Immunofluorescence
Microtubules
Microtubule associated proteins
Structured illumination microscopy
Online adgang:http://link.springer.com/article/10.1186/s13007-019-0406-z
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