Protocol for detecting histidine polyphosphate modification of human proteins via MBP-tagged expression in E. coli

Summary: Polyphosphate exhibits a unique post-translational modification-like function, known as histidine polyphosphate modification (HPM), marked by a robust non-covalent interaction with histidine repeat proteins. Here, we present a protocol for detecting HPM of human proteins via maltose-binding...

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Hauptverfasser:	Kirsten Lehotsky, Nolan Neville, Zongchao Jia
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Elsevier 2024-06-01
Schriftenreihe:	STAR Protocols
Schlagworte:	Molecular Biology Protein Biochemistry Protein expression and purification
Online-Zugang:	http://www.sciencedirect.com/science/article/pii/S2666166724001126
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