Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

Abstract Background Recently we proposed efficient method to exclude undesirable primers at any stage of amplification reaction, here termed NOPE (NOnsense-mediated Primer Exclusion). According to this method, added oligonucleotide overlapping with the 3′-end of unwanted amplification primer (NOPE o...

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Detalles Bibliográficos

Principais autores:	Dmitriy A. Shagin, Maria A. Turchaninova, Irina A. Shagina, Mikhail Shugay, Andrew R. Zaretsky, Olga I. Zueva, Dmitriy A. Bolotin, Sergey Lukyanov, Dmitriy M. Chudakov
Formato:	Artigo
Idioma:	Inglês
Publicado:	BMC 2017-06-01
Series:	BMC Genomics
Assuntos:	High-throughput sequencing Unique molecular identifiers Targeted resequencing PCR
Acceso en liña:	http://link.springer.com/article/10.1186/s12864-017-3815-2
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