Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering

Sleeping Beauty is used as a genome engineering tool in a range of organisms. Here, the authors solve an atomic structure of Sleeping Beauty (SB) transposase and model the target DNA into the active site, elucidating details that may enable the rational design of novel transposases.

Detalhes bibliográficos

Principais autores:	Franka Voigt, Lisa Wiedemann, Cecilia Zuliani, Irma Querques, Attila Sebe, Lajos Mátés, Zsuzsanna Izsvák, Zoltán Ivics, Orsolya Barabas
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Nature Portfolio 2016-03-01
coleção:	Nature Communications
Acesso em linha:	https://doi.org/10.1038/ncomms11126
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