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Fluorescent Protein Based FRET Pairs with Improved Dynamic Range for Fluorescence Lifetime Measurements.

Fluorescence Resonance Energy Transfer (FRET) using fluorescent protein variants is widely used to study biochemical processes in living cells. FRET detection by fluorescence lifetime measurements is the most direct and robust method to measure FRET. The traditional cyan-yellow fluorescent protein b...

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Autori principali: Bobin George Abraham, Karen S Sarkisyan, Alexander S Mishin, Ville Santala, Nikolai V Tkachenko, Matti Karp
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science (PLoS) 2015-01-01
Serie:PLoS ONE
Accesso online:https://doi.org/10.1371/journal.pone.0134436
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