Reduced Culture Temperature Differentially Affects Expression and Biophysical Properties of Monoclonal Antibody Variants

Reduced culture temperature is an increasingly popular practice to improve recombinant protein yields in CHO cells. Recent studies have attributed the enhancement of protein titers at sub-physiological temperatures to increased mRNA levels as well as extended stationary phase. We observed that reduc...

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Hlavní autoři:	Megan Mason, Bernadette Sweeney, Katharine Cain, Paul Stephens, Susan T. Sharfstein
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	MDPI AG 2014-08-01
Edice:	Antibodies
Témata:	Chinese hamster ovary cells monoclonal antibodies protein stability temperature shift antibody engineering
On-line přístup:	http://www.mdpi.com/2073-4468/3/3/253
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