Development of a CRISPR/Cas9-Based Tool for Gene Deletion in <italic toggle="yes">Issatchenkia orientalis</italic>

ABSTRACT The nonconventional yeast Issatchenkia orientalis has emerged as a potential platform microorganism for production of organic acids due to its ability to grow robustly under highly acidic conditions. However, lack of efficient genetic tools remains a major bottleneck in metabolic engineerin...

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Hauptverfasser:	Vinh G. Tran, Mingfeng Cao, Zia Fatma, Xiaofei Song, Huimin Zhao
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology 2019-06-01
Schriftenreihe:	mSphere
Schlagworte:	CRISPR/Cas9 Issatchenkia orientalis genome editing metabolic engineering synthetic biology
Online-Zugang:	https://journals.asm.org/doi/10.1128/mSphere.00345-19
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