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Full genome virus detection in fecal samples using sensitive nucleic acid preparation, deep sequencing, and a novel iterative sequence classification algorithm.

We have developed a full genome virus detection process that combines sensitive nucleic acid preparation optimised for virus identification in fecal material with Illumina MiSeq sequencing and a novel post-sequencing virus identification algorithm. Enriched viral nucleic acid was converted to double...

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Autors principals: Matthew Cotten, Bas Oude Munnink, Marta Canuti, Martin Deijs, Simon J Watson, Paul Kellam, Lia van der Hoek
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2014-01-01
Col·lecció:PLoS ONE
Accés en línia:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093269&type=printable
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