Protocol for Cas9-targeted long-read sequencing in Globodera pallida and Globodera rostochiensis
Summary: We present a protocol to achieve a higher depth of long-read sequencing of region(s) of interest in potato cyst nematodes without amplification using a Cas9-based Nanopore enrichment approach. We describe steps for designing high-fidelity guide RNAs to be used with Cas9 nuclease, extracting...
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| Principais autores: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2024-12-01
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| Serier: | STAR Protocols |
| Fag: | |
| Online adgang: | http://www.sciencedirect.com/science/article/pii/S2666166724005926 |
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